Tg(CAG-cat,-lacZ)11Miya
Transgene Detail

Summary

• Symbol: Tg(CAG-cat,-lacZ)11Miya
• Name: transgene insertion 11, Jun-ichi Miyazaki
• MGI ID: MGI:2176512
• Synonyms: CAG-CAT-Z, CAG-CAT-Z11, loxP-LlacZ, Tg(ACTB-cat-lacZ)11Miya
• Transgene: Tg(CAG-cat,-lacZ)11Miya Location: unknown
• Alliance: Tg(CAG-cat,-lacZ)11Miya page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: This allele is used to detect transient expression of Cre recombinase. The transgene carries a lacZ gene downstream of a CMV enhancer and chicken beta-actin promoter and a floxed chloramphenicol acetyltransferase gene (a stuffer sequence). Upon Cre-mediated excision of the stuffer sequence, the modified allele expresses lacZ in tissues where Cre is expressed. The authors state that a single copy of the transgene was inserted and expression was observed throughout all embryonic and adult stages. (J:69322)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The authors state that the strain of origin is "BDF1."

References

• Original: J:69322 Sakai K, et al., A transgenic mouse line that retains Cre recombinase activity in mature oocytes irrespective of the cre transgene transmission. Biochem Biophys Res Commun. 1997 Aug 18;237(2):318-24
• All: 61 reference(s)