Ptpn1^tm1Bpk
Targeted Allele Detail

Summary

• Symbol: Ptpn1^tm1Bpk
• Name: protein tyrosine phosphatase, non-receptor type 1; targeted mutation 1, Brian P Kennedy
• MGI ID: MGI:2176520
• Synonyms: PTP-1B -, PTP1B-KO
• Gene: Ptpn1 Location: Chr2:167773977-167821305 bp, + strand Genetic Position: Chr2, 87.99 cM, cytoband H3
• Alliance: Ptpn1^tm1Bpk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53437
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic fragment containing exons 5 and 6, which contain sequences encoding the tyrosine phospahatase active site. Western blot analysis on liver microsomes derived from homozygous mice confirmed that no detectable protein was expressed from this allele. (J:53437)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptpn1 Mutation: 36 strains or lines available

References

• Original: J:53437 Elchebly M, et al., Increased insulin sensitivity and obesity resistance in mice lacking the protein tyrosine phosphatase-1B gene [see comments]. Science. 1999 Mar 5;283(5407):1544-8
• All: 32 reference(s)