Ptpn11^tm1Paw
Targeted Allele Detail

Summary

• Symbol: Ptpn11^tm1Paw
• Name: protein tyrosine phosphatase, non-receptor type 11; targeted mutation 1, Anthony Pawson
• MGI ID: MGI:2176524
• Synonyms: Shp-2 -, Shp2 -, Shp-2^delta46-110
• Gene: Ptpn11 Location: Chr5:121268596-121329460 bp, - strand Genetic Position: Chr5, 61.72 cM, cytoband F
• Alliance: Ptpn11^tm1Paw page

Defects in the skin, lung and intestine of Egfr^wa2/Egfr^wa2 Ptpn11^tm1Paw/⁺ mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43740
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing exon 3, which contains sequences encoding the SH2-N motif. RT-PCR analysis on total RNA derived from heterozygous mice demonstrated that a transcript was made from this allele that spliced exon 2 to exon 4, resulting in a message that retained the proper reading frame. Western blot analysis on heterozygous specimins confirmed that a mutant protein is expressed from this allele that is consistent with the size expected. (J:43740)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptpn11 Mutation: 46 strains or lines available

References

• Original: J:43740 Saxton TM, et al., Abnormal mesoderm patterning in mouse embryos mutant for the SH2 tyrosine phosphatase Shp-2. EMBO J. 1997 May 1;16(9):2352-64
• All: 14 reference(s)