Egf^tm1Dle
Targeted Allele Detail

Summary

• Symbol: Egf^tm1Dle
• Name: epidermal growth factor; targeted mutation 1, David C Lee
• MGI ID: MGI:2176530
• Gene: Egf Location: Chr3:129471223-129548971 bp, - strand Genetic Position: Chr3, 58.5 cM
• Alliance: Egf^tm1Dle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54980
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing exon 20, which encodes the first two disulfide loops. Northern blot analysis on RNA derived from salivary gland and kidney of homozygous mice demonstrated that negligible amounts of transcipt is produced from this allele. Western blot analysis on samples derived from salivary gland of homozygous mice confirmed that no detectable protein is expressed from this allele. (J:54980)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Egf Mutation: 56 strains or lines available

References

• Original: J:54980 Luetteke NC, et al., Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development. Development. 1999 Jun;126(12):2739-50
• All: 11 reference(s)