Areg^tm1Dle
Targeted Allele Detail

Summary

• Symbol: Areg^tm1Dle
• Name: amphiregulin; targeted mutation 1, David C Lee
• MGI ID: MGI:2176531
• Synonyms: AR-
• Gene: Areg Location: Chr5:91287458-91296291 bp, + strand Genetic Position: Chr5, 44.78 cM
• Alliance: Areg^tm1Dle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54980
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing exons 3 and 4, which encode three disulfide loops, the heparin binding region and the transmembrane domain of the precursor protein. Northern blot analysis on RNA derived from stomach and skin of homozygous mice demonstrated that a smaller transcript is produced from this allele. RT-PCR and sequencing analysis confirmed the loss of coding sequences from this transcript. (J:54980)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Areg Mutation: 29 strains or lines available

References

• Original: J:54980 Luetteke NC, et al., Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development. Development. 1999 Jun;126(12):2739-50
• All: 43 reference(s)