Qui^DBA/2J
QTL Variant Detail

Summary

• QTL variant: Qui^DBA/2J
• Name: quinine sensitivity, taste; DBA/2J
• MGI ID: MGI:2176544
• QTL: Qui Location: Chr6:132546805-132549364 bp Genetic Position: Chr6, Syntenic

Variant origin

• Strain of Specimen: DBA/2J

Variant description

• Allele Type: QTL
• Inheritance: Other (see notes)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Qui exhibits additive inheritance.

Candidate Genes

J:4423

Qui, Rua, Cyx are linked to Prh1. Using 20 BXD and 7 CXB RI strains, no recombinants were detected between Qui and Prh1 (data not shown). Prp was orginally assigned to Chromosome 8, but later the assignment was corrected to Chromosome 6.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:12143

In 20 BXD RI strains and 7 CXB RI strains the strain distribution pattern for Prh1 and Qui was identical (95% confidence interval gives a genetic distance of 0-3.95 cM). Genes Rua and Cyx also followed this pattern. All four genes are very closely linked or may be identical.

J:75283

A QTL approach was used to map 2 previously identified loci involved with taste preference, Qui and Tas1r3 (previously referred to as Sac.) 61 male and 72 female (C57BL/6J x DBA/2J)F2 animals were screened for polymorphic markers on chromosomes 4 and 6 corresponding to the regions of Tas1r3 and Qui, respectively. Parental strain C57BL/6J shows stronger preference for sucrose, saccharin, and quinine solutions as shown by greater intake in a short term 1 bottle test compared to DBA/2J. A marker on mouse Chromosome 4 showed strong linkage to sucrose intake (LOD=8.85) and saccharin intake (LOD=10.61) at D4Mit42 (approximatlely 81 cM), mapping very close to Tas1r3. The C57BL/6J-derived allele appears to increase sucrose and saccharin intake in a dominant fashionat this locus. On mouse Chromosome 6, marker D6Mit338 (approximately 62.3 cM) was significantly linked to quinine intake (LOD=4.55) and maps close to Qui and Prh1. C57BL/6J-derived alleles appear to increase quinine intake in an additive fashion at thislocus.

J:8290

Thirty strains and substrains [Table 1] of mice were tested for their ability to taste a solution of raffinose undecaacetate (Rua). Variations in tasting were measured by the degree of aversion mice displayed towards the tastant. The inbred strains BALB/cBy and DBA/2 avoided tasting the Rua solution while C57BL/6J displayed little aversion to drinking the same solution.

An attempt to demonstrate the presence of a single gene with major effect on Rua tasting was explored using two sets of RI strains. 23 RI strains were used in total, 9 CxB strains (C=BALB/cBy; B=C57BL/6J) and 14 BXD strains (B=C57BL/6J; D=DBA/2J). Fourteen of the strains were non-tasters for Rua and nine were tasters, although the taster strains did not show quite the high degree of aversion as was shown by BALB/c and DBA/2, providing evidence for a locus with a major effect on RUA tasting, [Table 2]. The locus was assigned the symbol Rua, with the allelel Rua^a being present in strains BALB/c and DBA/2; and the allele Rua^b being present in C57BL/6J.

Both sets of RI strains can also be classified with respect to their sensitivity to the taste of quinine. The strain distribution pattern of Rua and Qui show complete concordance in 9 CXB (C=BALB/c; B=C57BL/6) recombinant inbred (RI) strains and in 14 BXD (B=C57BL/6; D=DBA/2) RI strain. [Table 2]. Rua and Qui appear tighly linked and segregate as a single locus.

References

• Original: J:75283 Blizard DA, et al., Quantitative trait loci associated with short-term intake of sucrose, saccharin and quinine solutions in laboratory mice. Chem Senses. 1999 Aug;24(4):373-85
• All: 1 reference(s)