Selplg^tm1Fur
Targeted Allele Detail

Summary

• Symbol: Selplg^tm1Fur
• Name: selectin, platelet (p-selectin) ligand; targeted mutation 1, Bruce Furie
• MGI ID: MGI:2176571
• Synonyms: PSGL-1-
• Gene: Selplg Location: Chr5:113956597-113970705 bp, - strand Genetic Position: Chr5, 55.59 cM
• Alliance: Selplg^tm1Fur page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75412
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a small DNA segment of the coding region (+69 to 94). A 300 bp segment in the 5' flanking region containing CT repeats was also deleted. Northern blot analysis on thymus and spleen RNA from homozygous mice demonstrated that no detecable transcript was produced from this allele. Flow cytometry experiments on leukocytes derived from homozygous mice confirmed that no detectable cell surface protein was expressed from this allele. (J:75412)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Selplg Mutation: 51 strains or lines available

References

• Original: J:75412 Yang J, et al., Targeted gene disruption demonstrates that P-selectin glycoprotein ligand 1 (PSGL-1) is required for P-selectin-mediated but not E-selectin-mediated neutrophil rolling and migration. J Exp Med. 1999 Dec 20;190(12):1769-82
• All: 56 reference(s)