Brca1^tm2Arge
Targeted Allele Detail

Summary

• Symbol: Brca1^tm2Arge
• Name: breast cancer 1, early onset; targeted mutation 2, Argiris Efstratiadis
• MGI ID: MGI:2177209
• Synonyms: Brca1^tr
• Gene: Brca1 Location: Chr11:101379590-101442781 bp, - strand Genetic Position: Chr11, 65.18 cM, cytoband D
• Alliance: Brca1^tm2Arge page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69187
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A loxP-flanked neomycin and thymidine kinase selection cassette was inserted into exon 11. The loxP flanked cassette was removed in ES cells by Cre mediated recombination prior to the production of chimeric mice, leaving a 50 bp insertion containing a loxP site. Northern blot analysis on RNA derived from total RNA of homozygous embryos demonstrated that aberrant transcripts were produced from this allele that either lacked exon 11, or contained exon 11 with the insertion, resulting in a frameshift mutation after the first 924 amino acids. This mutation was made to mimic a truncated protein associated with human breast cancer. (J:69187)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Brca1^tm2Arge

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brca1 Mutation: 114 strains or lines available

References

• Original: J:69187 Ludwig T, et al., Tumorigenesis in mice carrying a truncating Brca1 mutation. Genes Dev. 2001 May 15;15(10):1188-93
• All: 5 reference(s)