Brca2^tm1Cbl
Targeted Allele Detail

Summary

• Symbol: Brca2^tm1Cbl
• Name: breast cancer 2, early onset; targeted mutation 1, Chester Beatty Laboratories
• MGI ID: MGI:2177226
• Synonyms: Brca2^Tr2014
• Gene: Brca2 Location: Chr5:150446095-150493794 bp, + strand Genetic Position: Chr5, 89.52 cM
• Alliance: Brca2^tm1Cbl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44386
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 11 at position 6038. RT-PCR analysis on RNA derived from homozygous animals demonstrated that a partial transcript is made from this allele. Western blot analysis confirmed that no protein with an intact carboxy-terminus was expressed from this allele, but the authors do not rule out the possiblilty that a partial product could be made. (J:44386)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Brca2^tm1Cbl

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brca2 Mutation: 132 strains or lines available

References

• Original: J:44386 Connor F, et al., Tumorigenesis and a DNA repair defect in mice with a truncating Brca2 mutation. Nat Genet. 1997 Dec;17(4):423-30
• All: 2 reference(s)