E2f3^tm1Lees
Targeted Allele Detail

Summary

• Symbol: E2f3^tm1Lees
• Name: E2F transcription factor 3; targeted mutation 1, Jacqueline A Lees
• MGI ID: MGI:2177435
• Synonyms: E2F3-
• Gene: E2f3 Location: Chr13:30090558-30170046 bp, - strand Genetic Position: Chr13, 13.09 cM, cytoband A3.3
• Alliance: E2f3^tm1Lees page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65128
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced sequences encoding amino acids 134-294, which comprise the nuclear localization signal, cyclin A binding, DNA binding, and the leucine zipper domains, and introduced an in-frame termination codon just upstream of the cassette. Western blot analysis did not detect protein in MEF cells derived from homozygous mutant embryos. (J:65128)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying E2f3^tm1Lees

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any E2f3 Mutation: 33 strains or lines available

References

• Original: J:65128 Humbert PO, et al., E2f3 is critical for normal cellular proliferation. Genes Dev. 2000 Mar 15;14(6):690-703
• All: 15 reference(s)