Fus^{Gt(U3NeoSV1)HO14Ggh}
Gene trapped Allele Detail

Summary

• Symbol: Fus^{Gt(U3NeoSV1)HO14Ggh}
• Name: fused in sarcoma; gene trap HO14, Geoffrey G Hicks
• MGI ID: MGI:2177561
• Synonyms: Fus -, TLS^-
• Gene: Fus Location: Chr7:127565276-127581204 bp, + strand Genetic Position: Chr7, 69.87 cM
• Alliance: Fus^{Gt(U3NeoSV1)HO14Ggh} page

Mutation origin

• Mutant Cell Line: HO14
• Germline Transmission: Earliest citation of germline transmission: J:60213
• Parent Cell Line: D3H (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: U3NeoSV1
• Mutation details: The mutation was generated by the random insertion of the gene trap vector U3NeoSV1 into exon 12 of the gene. RT-PCR and Northern blot analysis demonstrated that a truncated transcript is produced from this allele, and western blot analysis confirmed that low levels of a truncated protein were expressed from this allele. However, the expressed product is not expected to retain activity due to missing nucleic acid binding domains. (J:60213)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fus Mutation: 48 strains or lines available

References

• Original: J:60213 Hicks GG, et al., Fus deficiency in mice results in defective B-lymphocyte development and activation, high levels of chromosomal instability and perinatal death. Nat Genet. 2000 Feb;24(2):175-9
• All: 8 reference(s)