Gad1^tm1Oba
Targeted Allele Detail

Summary

• Symbol: Gad1^tm1Oba
• Name: glutamate decarboxylase 1; targeted mutation 1, Kunihiko Obata
• MGI ID: MGI:2177565
• Synonyms: GAD67 -
• Gene: Gad1 Location: Chr2:70392109-70432358 bp, + strand Genetic Position: Chr2, 41.63 cM, cytoband D
• Alliance: Gad1^tm1Oba page

Gad1^tm1Oba/Gad1^tm1Oba mice and cleft palate

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:40978
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ gene followed by a neomycin resistance cassette was fused in frame in exon 1 of the gene. RT-PCR analysis on brain RNA derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on lysates derived from neonatal brain confirmed that no GAD1 protein is expressed from this allele. (J:40978)

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gad1 Mutation: 65 strains or lines available

References

• Original: J:40978 Asada H, et al., Cleft palate and decreased brain gamma-aminobutyric acid in mice lacking the 67-kDa isoform of glutamic acid decarboxylase. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6496-9
• All: 5 reference(s)