Gad2^tm1Oba
Targeted Allele Detail

Summary

• Symbol: Gad2^tm1Oba
• Name: glutamic acid decarboxylase 2; targeted mutation 1, Kunihiko Obata
• MGI ID: MGI:2177567
• Synonyms: GAD65 -
• Gene: Gad2 Location: Chr2:22512262-22583889 bp, + strand Genetic Position: Chr2, 15.15 cM, cytoband A-B
• Alliance: Gad2^tm1Oba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:37576
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 3 of the gene. Western blot analysis on brain extracts from homozygous mice confirmed that no detectable protein is expressed from this allele. (J:37576)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gad2 Mutation: 60 strains or lines available

References

• Original: J:37576 Asada H, et al., Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures. Biochem Biophys Res Commun. 1996 Dec 24;229(3):891-5
• All: 17 reference(s)