Gabra1^tm1Twr
Targeted Allele Detail

Summary

• Symbol: Gabra1^tm1Twr
• Name: gamma-aminobutyric acid type A receptor subunit alpha 1; targeted mutation 1, Thomas W Rosahl
• MGI ID: MGI:2177612
• Synonyms: alpha1 -, alpha1⁰
• Gene: Gabra1 Location: Chr11:42021766-42073757 bp, - strand Genetic Position: Chr11, 24.97 cM
• Alliance: Gabra1^tm1Twr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69290
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 4 and part of intron 4 was replaced with a loxP flanked neomycin resistance cassette. Northern blot analysis on RNA derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on brain lysates derived from homozygotes confirmed that no detectable protein is expressed from this allele. (J:69290)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabra1 Mutation: 48 strains or lines available

References

• Original: J:69290 Sur C, et al., Loss of the major GABA(A) receptor subtype in the brain is not lethal in mice. J Neurosci. 2001 May 15;21(10):3409-18
• All: 12 reference(s)