Eif2ak3^tm1Dron
Targeted Allele Detail

Summary

• Symbol: Eif2ak3^tm1Dron
• Name: eukaryotic translation initiation factor 2 alpha kinase 3; targeted mutation 1, David Ron
• MGI ID: MGI:2177666
• Synonyms: perk -
• Gene: Eif2ak3 Location: Chr6:70821499-70882229 bp, + strand Genetic Position: Chr6, 32.09 cM
• Alliance: Eif2ak3^tm1Dron page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70005
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced 1.6 kb of DNA containing the transmembrane domain. Northern blot analysis demonstrated that at least 10-fold less transcript was produced from this allele that the wild-type. However, western blot analysis on cells derived from homozygous mice confirmed that no detectable protein was expressed from this allele. (J:62257)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Eif2ak3 Mutation: 53 strains or lines available

References

• Original: J:62257 Harding HP, et al., Perk is essential for translational regulation and cell survival during the unfolded protein response. Mol Cell. 2000 May;5(5):897-904
• All: 29 reference(s)