Gck^tm1.2Mgn
Targeted Allele Detail

Summary

• Symbol: Gck^tm1.2Mgn
• Name: glucokinase; targeted mutation 1.2, Mark A Magnuson
• MGI ID: MGI:2177710
• Synonyms: gk^del
• Gene: Gck Location: Chr11:5850820-5900081 bp, - strand Genetic Position: Chr11, 3.88 cM
• Alliance: Gck^tm1.2Mgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51826
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele is a derivative of Gck^tm1Mgn in which all the loxP flanked sequences including exons 9 and 10 as well as the neomycin selection cassette were deleted by injecting heterozygous fertilized eggs with a plasmid expressing Cre recombinase. Progeny were screened for the allele in which all sequences were deleted. (J:51826)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1  Disease Model	Gck^tm1.2Mgn/Gck^tm1.2Mgn	involves: 129S6/SvEvTac * C57BL/6
ht2  Disease Model	Gck^tm1.2Mgn/Gck^+	involves: 129S6/SvEvTac * C57BL/6

Phenotypes:

Affected Systems	hm1	ht2
show or hide all annotated terms
endocrine/exocrine glands		√
decreased insulin secretion		√
homeostasis/metabolism	√	√
decreased insulin secretion		√
hyperglycemia	√	√
decreased circulating insulin level	√
increased circulating free fatty acids level	√
impaired glucose tolerance		√
decreased liver glycogen level	√	√
liver/biliary system	√	√
decreased liver glycogen level	√	√
hepatic steatosis	√	√
mortality/aging	√
postnatal lethality, complete penetrance	√

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Key:

√

disease model

expected model not found

Models:

Human Diseases	hm1	ht2
maturity-onset diabetes of the young type 2 IDs maturity-onset diabetes of the young type 2       DOID:0111100 OMIM:125851 Close	√	√

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gck Mutation: 64 strains or lines available

References

• Original: J:51826 Postic C, et al., Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. J Biol Chem. 1999 Jan 1;274(1):305-15
• All: 2 reference(s)