Lif^tm1Stw
Targeted Allele Detail

Summary

• Symbol: Lif^tm1Stw
• Name: leukemia inhibitory factor; targeted mutation 1, Colin L Stewart
• MGI ID: MGI:2177780
• Synonyms: L-, Lif-, LIFKO
• Gene: Lif Location: Chr11:4207587-4222514 bp, + strand Genetic Position: Chr11, 2.94 cM, cytoband A1-A2
• Alliance: Lif^tm1Stw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:35071
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance gene was inserted into exon 3. Northern blot analysis on embryonic fibroblasts isolated from homozygous embryos demonstrated that no detectable transcript was produced from this allele, and cell growth assays confirmed that no functional protein was expressed from this allele. (J:35071)

Expression

In Mice Carrying this Mutation:	42 assay results
In Structures Affected by this Mutation:	18 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lif Mutation: 11 strains or lines available

References

• Original: J:35071 Stewart CL, et al., Blastocyst implantation depends on maternal expression of leukaemia inhibitory factor [see comments]. Nature. 1992 Sep 3;359(6390):76-9
• All: 34 reference(s)