Kmt2a^tm1Vss
Targeted Allele Detail

Summary

• Symbol: Kmt2a^tm1Vss
• Name: lysine (K)-specific methyltransferase 2A; targeted mutation 1, Vasanta Subramanian
• MGI ID: MGI:2177819
• Gene: Kmt2a Location: Chr9:44714652-44792594 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Kmt2a^tm1Vss page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71498
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An IRES-lacZ gene followed by a neomycin selection cassette was inserted into exon 5. RT-PCR analysis confirmed the expression of a mutant transcipt, and whole mount staining of embryos demonstrated that beta-galactosidase was expressed from this allele. (J:71498)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmt2a Mutation: 135 strains or lines available

References

• Original: J:71498 Ayton P, et al., Truncation of the MLL gene in exon 5 by gene targeting leads to early preimplantation lethality of homozygous embryos. Genesis. 2001 Aug;30(4):201-12
• All: 1 reference(s)