Itgb3^tm1Drp
Targeted Allele Detail

Summary

• Symbol: Itgb3^tm1Drp
• Name: integrin beta 3; targeted mutation 1, David R Phillips
• MGI ID: MGI:2177827
• Synonyms: beta3 diYF, DiYF
• Gene: Itgb3 Location: Chr11:104498826-104561302 bp, + strand Genetic Position: Chr11, 67.84 cM
• Alliance: Itgb3^tm1Drp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73416
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Point mutations were introduced by homologous recombination that altered codons 747 and 759 from ones encoding tyrosine to ones encoding phenylalanine. An FRT-flanked neomycin selection cassette that was inserted into an adjacent intron was removed in ES cells by Flp-mediated recombination in ES cells prior to the production of chimeric mice, leaving a single FRT site in place of the selection cassette. Western blot analysis and flow cytometric analysis on platelets derived from homozygous mice confirmed that a mutant protein was expressed on the cell surface. (J:73416)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itgb3 Mutation: 45 strains or lines available

References

• Original: J:73416 Law DA, et al., Integrin cytoplasmic tyrosine motif is required for outside-in alphaIIbbeta3 signalling and platelet function. Nature. 1999 Oct 21;401(6755):808-11
• All: 6 reference(s)