F2^tm1Sjd
Targeted Allele Detail

Summary

• Symbol: F2^tm1Sjd
• Name: coagulation factor II; targeted mutation 1, Sandra JF Degen
• MGI ID: MGI:2177948
• Synonyms: FII-
• Gene: F2 Location: Chr2:91455665-91466759 bp, - strand Genetic Position: Chr2, 50.63 cM
• Alliance: F2^tm1Sjd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48273
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An HPRT minigene replaced a genomic fragment containing exons 1 and 2, which encode the signal peptide and pro-peptide. Northern blot analysis on liver RNA derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on liver extracts derived from plasma of homozygous mice confirmed that no protein was expressed from this allele. (J:48273)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F2 Mutation: 36 strains or lines available

References

• Original: J:48273 Sun WY, et al., Prothrombin deficiency results in embryonic and neonatal lethality in mice. Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7597-602
• All: 6 reference(s)