F2^tm1Jes
Targeted Allele Detail

Summary

• Symbol: F2^tm1Jes
• Name: coagulation factor II; targeted mutation 1, J Evan Sadler
• MGI ID: MGI:2177949
• Synonyms: Cf2-
• Gene: F2 Location: Chr2:91455665-91466759 bp, - strand Genetic Position: Chr2, 50.63 cM
• Alliance: F2^tm1Jes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48274
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic DNA fragment that contains exons 7-11, which encode one of the kringle domains, the activation cleavage site and part of the active site. Western blot analysis on liver extracts derived from homozygous mice confirmed that no detectable protein was expressed from this allele. (J:48274)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F2 Mutation: 36 strains or lines available

References

• Original: J:48274 Xue J, et al., Incomplete embryonic lethality and fatal neonatal hemorrhage caused by prothrombin deficiency in mice. Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7603-7
• All: 2 reference(s)