Srebf1^tm2Jdh
Targeted Allele Detail

Summary

• Symbol: Srebf1^tm2Jdh
• Name: sterol regulatory element binding transcription factor 1; targeted mutation 2, Jay D Horton
• MGI ID: MGI:2177958
• Synonyms: allele B
• Gene: Srebf1 Location: Chr11:60089915-60113407 bp, - strand Genetic Position: Chr11, 37.81 cM
• Alliance: Srebf1^tm2Jdh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43570
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment extending from the middle of exon 5 through exon 11. Western blot analysis on membrane and nuclear extracts of liver from homozygous mice demonstrated that a truncated protein was expressed from this allele that lacked the DNA-binding domains. (J:43570)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Srebf1 Mutation: 55 strains or lines available

Notes

ES cell line = JH1 (129S7/SvEvBrd) or SM1 (129S6/SvEvTac).

References

• Original: J:43570 Shimano H, et al., Elevated levels of SREBP-2 and cholesterol synthesis in livers of mice homozygous for a targeted disruption of the SREBP-1 gene [see comments]. J Clin Invest. 1997 Oct 15;100(8):2115-24
• All: 5 reference(s)