Krt8^tm1Rgo
Targeted Allele Detail

Summary

• Symbol: Krt8^tm1Rgo
• Name: keratin 8; targeted mutation 1, Robert G Oshima
• MGI ID: MGI:2178042
• Synonyms: K8^-, mK8 -
• Gene: Krt8 Location: Chr15:101905146-101912777 bp, - strand Genetic Position: Chr15, 57.2 cM
• Alliance: Krt8^tm1Rgo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:12899
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a DNA segment containing most of the first exon including the translation initiation codon. Northern blot analysis on RNA isolated from gut, liver, kidney and spleen demonstrated that an aberrant transcript was made in gut, but no transcript was detectable elsewhere. Immunohistochemistry on sections of intestine of homozygous mice confirmed that no detectable protein was expressed from this allele. (J:12899)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Krt8^tm1Rgo

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Krt8 Mutation: 34 strains or lines available

References

• Original: J:12899 Baribault H, et al., Mid-gestational lethality in mice lacking keratin 8. Genes Dev. 1993 Jul;7(7A):1191-202
• All: 36 reference(s)