F3^tm1Dwr
Targeted Allele Detail

Summary

• Symbol: F3^tm1Dwr
• Name: coagulation factor III; targeted mutation 1, Mieke Dewerchin
• MGI ID: MGI:2178047
• Synonyms: TF^neo
• Gene: F3 Location: Chr3:121517186-121528697 bp, + strand Genetic Position: Chr3, 52.94 cM
• Alliance: F3^tm1Dwr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71271
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 6, encoding the last 18 amino acids, was deleted. In addition, a loxP-flanked neomycin resistance cassette was inserted into exon 5. Northen blot analysis confirmed the expression of a fusion transcript with the 3'UTR and neo cassette. (J:71271)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F3 Mutation: 24 strains or lines available

References

• Original: J:71271 Melis E, et al., Targeted deletion of the cytosolic domain of tissue factor in mice does not affect development. Biochem Biophys Res Commun. 2001 Aug 24;286(3):580-6
• All: 2 reference(s)