Grid2^tm1Mim
Targeted Allele Detail

Summary

• Symbol: Grid2^tm1Mim
• Name: glutamate receptor, ionotropic, delta 2; targeted mutation 1, Masayoshi Mishina
• MGI ID: MGI:2178052
• Synonyms: delta2-, GluRdelta2-, GluRdelta2 mutant
• Gene: Grid2 Location: Chr6:63232860-64681307 bp, + strand Genetic Position: Chr6, 29.77 cM
• Alliance: Grid2^tm1Mim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:24975
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene replaced a genomic DNA fragment containing exon M3, which encodes a putative transmembrane segment. In situ hybridization analysis on brain of homozygous mice demonstrated that no normal transcript was produced from this allele. Western blot analysis on total cerebellar proteins derived from homozygous mice confirmed that no detectable protein was expressed from this allele. (J:24975)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Grid2 Mutation: 85 strains or lines available

References

• Original: J:24975 Kashiwabuchi N, et al., Impairment of motor coordination, Purkinje cell synapse formation, and cerebellar long-term depression in GluR delta 2 mutant mice. Cell. 1995 Apr 21;81(2):245-52
• All: 26 reference(s)