Kit^tm1.1Bsm
Targeted Allele Detail

Summary

• Symbol: Kit^tm1.1Bsm
• Name: KIT proto-oncogene receptor tyrosine kinase; targeted mutation 1.1, Peter Besmer
• MGI ID: MGI:2178059
• Synonyms: kit^Y719F
• Gene: Kit Location: Chr5:75735647-75817382 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Kit^tm1.1Bsm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61141
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: This allele is a derivative of Kit^tm1Bsm in which a Cre mediated recombination event removed the neomycin cassette. Excision of the neomycin cassette restores normal Kit expression. The resulting heritable mutation, Y719F, was designed to prevent the binding of SH2 domain proteins including the p85 subunit of the PI 3-kinase and to abolish subsequent signaling events. (J:61141)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kit Mutation: 182 strains or lines available

References

• Original: J:61141 Kissel H, et al., Point mutation in kit receptor tyrosine kinase reveals essential roles for kit signaling in spermatogenesis and oogenesis without affecting other kit responses. EMBO J. 2000 Mar 15;19(6):1312-26
• All: 8 reference(s)