Mycl^tm1Rdp
Targeted Allele Detail

Summary

• Symbol: Mycl^tm1Rdp
• Name: v-myc avian myelocytomatosis viral oncogene lung carcinoma derived; targeted mutation 1, Ronald DePinho
• MGI ID: MGI:2178221
• Synonyms: L-myc -
• Gene: Mycl Location: Chr4:122889445-122896278 bp, + strand Genetic Position: Chr4, 57.36 cM
• Alliance: Mycl^tm1Rdp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:31946
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 19 bp oligonucleotide containing a restriction site and a translation termination codon was inserted into exon 2. In addition, part of exon 3 was replaced with a neomycin resistance cassette. Northern blot and western blot analysis confirmed that no detectable transcript or protein was expressed from this allele. (J:31946)

Expression

In Mice Carrying this Mutation:

49 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mycl Mutation: 19 strains or lines available

References

• Original: J:31946 Hatton KS, et al., Expression and activity of L-Myc in normal mouse development. Mol Cell Biol. 1996 Apr;16(4):1794-804
• All: 1 reference(s)