Hells^tm1Kmu
Targeted Allele Detail

Summary

• Symbol: Hells^tm1Kmu
• Name: helicase, lymphoid specific; targeted mutation 1, Kathrin Muegge
• MGI ID: MGI:2178586
• Synonyms: Lsh -
• Gene: Hells Location: Chr19:38919359-38959495 bp, + strand Genetic Position: Chr19, 33.57 cM, cytoband C3-D1
• Alliance: Hells^tm1Kmu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76112
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 6 and 7, containing a helicase domain (ATPase), and a DExH box, were relaced with a PGK-neo cassette. Successful targeting was confirmed by Southern analysis and PCR. Western analysis failed to detect protein from embryonic liver extracts in homozygous mutant animals. (J:76112)

Expression

In Mice Carrying this Mutation:	41 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hells Mutation: 58 strains or lines available

References

• Original: J:76112 Geiman TM, et al., Lsh, a SNF2 family member, is required for normal murine development. Biochim Biophys Acta. 2001 May 3;1526(2):211-20
• All: 12 reference(s)