Crhr2^tm1Jsp
Targeted Allele Detail

Summary

• Symbol: Crhr2^tm1Jsp
• Name: corticotropin releasing hormone receptor 2; targeted mutation 1, Joachim Speiss
• MGI ID: MGI:2178616
• Synonyms: Crhr2^-
• Gene: Crhr2 Location: Chr6:55067034-55110001 bp, - strand Genetic Position: Chr6, 27.33 cM
• Alliance: Crhr2^tm1Jsp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61485
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic fragment containing exons which encode the third intracelllular loop. Binding assays on sections of adult homozygous brain confirmed that no functional protein was expressed from this allele. No detectable transcript was produced from this allele (data not shown). (J:61485)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Crhr2^tm1Jsp/Crhr2^tm1Jsp	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
ht2	Crhr2^tm1Jsp/Crhr2^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

Phenotypes:

Affected Systems	hm1	ht2
show or hide all annotated terms
behavior/neurological	√	√
behavior/neurological phenotype	N	N
increased anxiety-related response	√	√
cardiovascular system	√
abnormal vascular permeability	√
hearing/vestibular/ear	N
hearing/vestibular/ear phenotype	N
homeostasis/metabolism	N	N
homeostasis/metabolism phenotype	N	N
vision/eye	N
vision/eye phenotype	N

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Crhr2 Mutation: 35 strains or lines available

References

• Original: J:61485 Kishimoto T, et al., Deletion of crhr2 reveals an anxiolytic role for corticotropin-releasing hormone receptor-2. Nat Genet. 2000 Apr;24(4):415-9
• All: 3 reference(s)