Otx2^tm4Sia
Targeted Allele Detail

Summary

• Symbol: Otx2^tm4Sia
• Name: orthodenticle homeobox 2; targeted mutation 4, Shinichi Aizawa
• MGI ID: MGI:2178747
• Synonyms: Otx2^frt-neo
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^tm4Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74631
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The promoter and first exon of the gene were flanked with inserted loxP sites. In addition, an frt-flanked neomycin resistance cassette was inserted into first intron. RT-PCR analysis on RNA derived from homozygous embryos demonstrated that aberrant neo-fusion transcripts were produced from this allele in addition to reduced levels of normal mRNA. (J:74631)

Expression

In Mice Carrying this Mutation:	42 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:74631 Tian E, et al., Otx2 is required to respond to signals from anterior neural ridge for forebrain specification. Dev Biol. 2002 Feb 15;242(2):204-23
• All: 1 reference(s)