Itk^tm1Litt
Targeted Allele Detail

Summary

• Symbol: Itk^tm1Litt
• Name: IL2 inducible T cell kinase; targeted mutation 1, Dan R Littman
• MGI ID: MGI:2178820
• Synonyms: itk^-, Itk^tm1Drl
• Gene: Itk Location: Chr11:46215977-46280342 bp, - strand Genetic Position: Chr11, 27.75 cM
• Alliance: Itk^tm1Litt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:31230
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4 and part of exon 3 of this gene was replaced with a PGK(G)neopA cassette via homologous recombination. A stop codon was engineered at position 78 to further ensure a null allele. PCR and Southern blot analysis confirmed the presence of the targeted mutation, and Western blot analysis determined no Itk protein was present in thymocytes of homozygous mutant animals. (J:31230)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Itk Mutation: 50 strains or lines available

References

• Original: J:31230 Liao XC, et al., Altered T cell receptor signaling and disrupted T cell development in mice lacking Itk. Immunity. 1995 Dec;3(6):757-69
• All: 53 reference(s)