Itk^tm1Ljb
Targeted Allele Detail

Summary

• Symbol: Itk^tm1Ljb
• Name: IL2 inducible T cell kinase; targeted mutation 1, Leslie Berg
• MGI ID: MGI:2178928
• Synonyms: Itk-
• Gene: Itk Location: Chr11:46215977-46280342 bp, - strand Genetic Position: Chr11, 27.75 cM
• Alliance: Itk^tm1Ljb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48231
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The kinase domain of this gene (amino acids 476-543) spanning from the middle of exon 10 to exon 12 was replaced with a PGK-neo cassette via homologous recombination. Protein product was absent in lymph nodes, spleen, and thymus of homozygous mutant animals via Western blot analysis. (J:48231)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itk Mutation: 50 strains or lines available

Notes

J1 and CCE were used in the generation of this targeted mutation.

References

• Original: J:48231 Liu KQ, et al., T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells. J Exp Med. 1998 May 18;187(10):1721-7
• All: 23 reference(s)