Cd44^tm2Kmz
Targeted Allele Detail

Summary

• Symbol: Cd44^tm2Kmz
• Name: CD44 antigen; targeted mutation 2, Katalin Mikecz
• MGI ID: MGI:2178950
• Synonyms: CD44-
• Gene: Cd44 Location: Chr2:102641486-102732010 bp, - strand Genetic Position: Chr2, 54.13 cM
• Alliance: Cd44^tm2Kmz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74268
• Parent Cell Line: DBA-252 (ES Cell)
• Strain of Origin: DBA/1LacJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene replaced most of exons 4 and 5 of this gene (encoding part of the hyaluronan binding domain and IM7 mAB recognition site) via homologous recombination. PCR and Southern Blot confirmed the integration of the neo gene in homozygous mutant animals. Synovial fibroblasts from homozygous mutant animals did not express mRNA as determined by Northern blot, and Western blot using IM7 mAB as a probe yielded negative results. (J:74268)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd44 Mutation: 72 strains or lines available

References

• Original: J:74268 Stoop R, et al., Increased resistance to collagen-induced arthritis in CD44-deficient DBA/1 mice. Arthritis Rheum. 2001 Dec;44(12):2922-31
• All: 10 reference(s)