Grin2b^tm2Mim
Targeted Allele Detail

Summary

• Symbol: Grin2b^tm2Mim
• Name: glutamate receptor, ionotropic, NMDA2B (epsilon 2); targeted mutation 2, Masayoshi Mishina
• MGI ID: MGI:2179059
• Synonyms: GluRepsilon2dC
• Gene: Grin2b Location: Chr6:135690231-136150509 bp, - strand Genetic Position: Chr6, 66.38 cM
• Alliance: Grin2b^tm2Mim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76019
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A termination codon and a neomycin selection cassette were replaced 0.9 kb of the sequences following codon 997. RNAase protection assays on RNA derived from brain of homozyous mice demonstrated that normal levels of a mutant transcript are produced from this allele. Western blot analysis on whole brain lysates confirmed that a truncated protein is expressed from this allele in homozygous mice. (J:76019)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grin2b Mutation: 99 strains or lines available

References

• Original: J:76019 Mori H, et al., Role of the carboxy-terminal region of the GluR epsilon2 subunit in synaptic localization of the NMDA receptor channel. Neuron. 1998 Sep;21(3):571-80
• All: 1 reference(s)