Ppm1d^tm1Lad
Targeted Allele Detail

Summary

• Symbol: Ppm1d^tm1Lad
• Name: protein phosphatase 1D magnesium-dependent, delta isoform; targeted mutation 1, Lawrence A Donehower
• MGI ID: MGI:2179128
• Synonyms: Wip1-, WIP1^-
• Gene: Ppm1d Location: Chr11:85202080-85237897 bp, + strand Genetic Position: Chr11, 51.34 cM
• Alliance: Ppm1d^tm1Lad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74284
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 4 and 5 were replaced with a PGK-puro cassette via homologous recombination resulting in protein truncation. Northern blot analysis of tissues from homozygous mutant animals did not detect gene transcript although a transcript with deleted exons 4 and 5, presumed to be unstable, was detected by RT-PCR. Western blot of irradiated testes from homozygous mutants did not detect protein product. (J:74284)

Expression

In Mice Carrying this Mutation:	1 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ppm1d Mutation: 31 strains or lines available

References

• Original: J:74284 Choi J, et al., Mice deficient for the wild-type p53-induced phosphatase gene (Wip1) exhibit defects in reproductive organs, immune function, and cell cycle control. Mol Cell Biol. 2002 Feb;22(4):1094-105
• All: 30 reference(s)