Fyn^tm1Rmp
Targeted Allele Detail

Summary

• Symbol: Fyn^tm1Rmp
• Name: Fyn proto-oncogene; targeted mutation 1, Roger M Perlmutter
• MGI ID: MGI:2179145
• Synonyms: fynT, p59^fyn, p59fynT
• Gene: Fyn Location: Chr10:39245735-39441377 bp, + strand Genetic Position: Chr10, 20.51 cM
• Alliance: Fyn^tm1Rmp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70886
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo-derived cassette was used to replace exon 7b via homologous recombination, disrupting the thymic isoform of this gene while leaving the brain isoform intact. Western blot and immunoprecipitation did not detect protein product in thymocytes or splenocytes of homozygous mutant animals but protein product was present in brain. In Northern blot, mutant transcripts were detected in thymus whereas wild-type transcripts were detected in brain, proving successful knock out of the thymic isoform. (J:70886)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fyn Mutation: 39 strains or lines available

Notes

ES cell line = D3J8.

References

• Original: J:70886 Appleby MW, et al., Defective T cell receptor signaling in mice lacking the thymic isoform of p59fyn. Cell. 1992 Sep 4;70(5):751-63
• All: 18 reference(s)