Lig1^tm2Dwm
Targeted Allele Detail

Summary

• Symbol: Lig1^tm2Dwm
• Name: ligase I, DNA, ATP-dependent; targeted mutation 2, David W Melton
• MGI ID: MGI:2179196
• Synonyms: Lig1^#12, Lig1^-(no.12)
• Gene: Lig1 Location: Chr7:13011239-13045350 bp, + strand Genetic Position: Chr7, 7.82 cM
• Alliance: Lig1^tm2Dwm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75962
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 3' end of the gene (exons 23-27) has been replaced with an HPRT minigene. In this allele, the more highly expressed DWM110 minigene was utilized. RT-PCR detected levels of Lig1-Hprt fusion transcripts although no protein product was detected by Western analysis. (J:75962)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lig1 Mutation: 48 strains or lines available

References

• Original: J:75962 Bentley DJ, et al., DNA ligase I null mouse cells show normal DNA repair activity but altered DNA replication and reduced genome stability. J Cell Sci. 2002 Apr 1;115(Pt 7):1551-61
• All: 2 reference(s)