Top2b^tm1Jcw
Targeted Allele Detail

Summary

• Symbol: Top2b^tm1Jcw
• Name: topoisomerase (DNA) II beta; targeted mutation 1, James C Wang
• MGI ID: MGI:2179201
• Synonyms: top2beta -/-, top2beta^delta1
• Gene: Top2b Location: Chr14:6038976-6104585 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A3
• Alliance: Top2b^tm1Jcw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75958
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two exons of the endogenous locus, one of which contains the active-site tyrosine codon, were replaced by a neo cassette. No transcript was detected from the liver of E18.5 mutant embryos. No protein was detected in extracts from fibroblasts of E13.5 mutant embryos. (J:75958)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Top2b Mutation: 91 strains or lines available

References

• Original: J:75958 Yang X, et al., DNA topoisomerase IIbeta and neural development. Science. 2000 Jan 7;287(5450):131-4
• All: 6 reference(s)