Capns1^tm1Pag
Targeted Allele Detail

Summary

• Symbol: Capns1^tm1Pag
• Name: calpain, small subunit 1; targeted mutation 1, Peter A Greer
• MGI ID: MGI:2179226
• Synonyms: Capn4-
• Gene: Capns1 Location: Chr7:29886361-29898236 bp, - strand Genetic Position: Chr7, 17.31 cM
• Alliance: Capns1^tm1Pag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74966
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Disruption of the calpain small subunit gene was achieved by replacing the 3'-terminal half of exon 9 with a PGK-neo cassette. No calpain activity was detected in extracts from homozygous mutant ES cells. Although the large subunit genes were not altered and are transcribed, the resultant proteins are unstable in the absence of the small subunit. (J:74966)

Expression

In Mice Carrying this Mutation:

21 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Capns1 Mutation: 34 strains or lines available

References

• Original: J:74966 Arthur JS, et al., Disruption of the murine calpain small subunit gene, Capn4: calpain is essential for embryonic development but not for cell growth and division. Mol Cell Biol. 2000 Jun;20(12):4474-81
• All: 4 reference(s)