Lig1^tm1Dwm
Targeted Allele Detail

Summary

• Symbol: Lig1^tm1Dwm
• Name: ligase I, DNA, ATP-dependent; targeted mutation 1, David W Melton
• MGI ID: MGI:2179227
• Synonyms: Lig1^#53
• Gene: Lig1 Location: Chr7:13011239-13045350 bp, + strand Genetic Position: Chr7, 7.82 cM
• Alliance: Lig1^tm1Dwm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76371
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 3' end of the gene (exons 23-27) was replaced with a PGK-HPRT minigene. Northern analysis failed to detect any intact, truncated, or fusion transcripts in homozygous mutant embryos. Western analysis failed to detect any protein in homozygous mutant embryos, when probed with antibodies against bovine DNA ligase 1. (J:76371)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lig1 Mutation: 48 strains or lines available

References

• Original: J:76371 Bentley D, et al., DNA ligase I is required for fetal liver erythropoiesis but is not essential for mammalian cell viability. Nat Genet. 1996 Aug;13(4):489-91
• All: 2 reference(s)