Polm^tm1Crey
Targeted Allele Detail

Summary

• Symbol: Polm^tm1Crey
• Name: polymerase (DNA directed), mu; targeted mutation 1, Claude-Agnes Reynaud
• MGI ID: MGI:2179233
• Synonyms: pol mu-
• Gene: Polm Location: Chr11:5777860-5788016 bp, - strand Genetic Position: Chr11, 3.87 cM
• Alliance: Polm^tm1Crey page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75927
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 7-9 were replaced with a neomycin resistance gene via homologous recombination resulting in truncation of exons 6 and 10 and loss of most of the polX domain. RT-PCR of spleen B cells from homozygous mutant animals failed to detect gene expression. (J:75927)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Polm Mutation: 29 strains or lines available

References

• Original: J:75927 Bertocci B, et al., Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation. J Immunol. 2002 Apr 15;168(8):3702-6
• All: 3 reference(s)