Dffa^tm1Mxu
Targeted Allele Detail

Summary

• Symbol: Dffa^tm1Mxu
• Name: DNA fragmentation factor, alpha subunit; targeted mutation 1, Ming Xu
• MGI ID: MGI:2179456
• Synonyms: DFF45 -
• Gene: Dffa Location: Chr4:149188603-149205104 bp, + strand Genetic Position: Chr4, 78.87 cM
• Alliance: Dffa^tm1Mxu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50707
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing the first three exons of the gene. Western blot analysis on extracts of various tissues derived from homozygous mice confirmed that no protein was expressed from this allele. (J:50707)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dffa Mutation: 16 strains or lines available

References

• Original: J:50707 Zhang J, et al., Resistance to DNA fragmentation and chromatin condensation in mice lacking the DNA fragmentation factor 45. Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12480-5
• All: 8 reference(s)