Or10a4^{tm2(Or7a40)Mom}
Targeted Allele Detail

Summary

• Symbol: Or10a4^{tm2(Or7a40)Mom}
• Name: olfactory receptor family 10 subfamily A member 4; targeted mutation 2, Peter Mombaerts
• MGI ID: MGI:2179504
• Synonyms: M12->P2-IRES-tau-lacZ, Or10a4^{tm2(Olfr19)Mom}
• Gene: Or10a4 Location: Chr7:106696674-106697621 bp, + strand Genetic Position: Chr7, 56.1 cM, cytoband F2
• Alliance: Or10a4^{tm2(Or7a40)Mom} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36595
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The coding region for Olfr19, modified by introduction of the hemagglutinin epitope tag between the second and third exon, was linked to the 5' end of an IRES-tau-lacZ-loxP-tk-neo-loxP expression cassette. Insertion of this construct replaced the entire translated region of the endogenous gene, and placed expression of the inserted sequences under control of the endogenous regulatory sequences. Transient expression of Cre recombinase in C57BL/6 blastocysts excised the thymidine kinase and neomycin sequences. This allele expresses a bicistronic mRNA encoding Olfr19 and a bovine tau-beta-galactosidase fusion protein. (J:36595)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Or10a4 Mutation: 22 strains or lines available

References

• Original: J:36595 Mombaerts P, et al., Visualizing an olfactory sensory map. Cell. 1996 Nov 15;87(4):675-86
• All: 1 reference(s)