Relb^{Tg(H2-K1/GH1)106Bri}
Transgenic Allele Detail

Summary

• Symbol: Relb^{Tg(H2-K1/GH1)106Bri}
• Name: avian reticuloendotheliosis viral (v-rel) oncogene related B; transgene insertion 106, Ralph L Brinster
• MGI ID: MGI:2179544
• Synonyms: 272H, line 272-4, relb^-, Relb^Tg106Bri, Relb^{Tg(H2K/GH1)106Bri}, Tg(H-2K + GH1), Tg(H-2K +GH1)Bri106, Tg(H2KhGH1)106Bri
• Gene: Relb Location: Chr7:19340142-19363352 bp, - strand Genetic Position: Chr7, 9.93 cM
• Alliance: Relb^{Tg(H2-K1/GH1)106Bri} page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: The mutation was generated by microinjection of a construct containing a 7.8 kb fragment that includes the complete mouse H2-K structural locus and 2 kb of 5' flanking sequence, as well as a nonfunctional 2.1 kb portion of the human growth hormone gene (hGH1), which serves as a marker gene. The random insertion of this transgene just downstream of the exon encoding the Rel domain disrupted the gene. RT-PCR analysis of RNA derived from slpeen and thymus of homozygous mice demonstrated that no detectable transcript was expressed from this allele. Immunohistochemistry experiments on sections of thymus and spleen of homozygous mice confirmed that no detectable encoded protein was present. (J:23081)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Relb Mutation: 33 strains or lines available

References

• Original: J:76450 Lo D, et al., A recessive defect in lymphocyte or granulocyte function caused by an integrated transgene. Am J Pathol. 1992 Nov;141(5):1237-46
• All: 29 reference(s)