Kcnab2^tm2Mes
Targeted Allele Detail

Summary

• Symbol: Kcnab2^tm2Mes
• Name: potassium voltage-gated channel, shaker-related subfamily, beta member 2; targeted mutation 2, Albee Messing
• MGI ID: MGI:2179739
• Synonyms: Kvbeta2-Y90F
• Gene: Kcnab2 Location: Chr4:152475201-152561991 bp, - strand Genetic Position: Chr4, 83.08 cM
• Alliance: Kcnab2^tm2Mes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75891
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A tyrosine to phenylalanine substitution at amino acid 90 (p.Y90F) was introduced via homologous recombination. The resulting protein product is enzymatically inactive yet retains structural integrity and DNA binding ability. The Y90F point mutation was verified via RT-PCR and restriction digest analysis of RNA transcripts from mutant animals. (J:75891)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kcnab2 Mutation: 24 strains or lines available

References

• Original: J:75891 McCormack K, et al., Genetic analysis of the mammalian K+ channel beta subunit Kvbeta 2 (Kcnab2). J Biol Chem. 2002 Apr 12;277(15):13219-28
• All: 2 reference(s)