Ank3^tm1Bnt
Targeted Allele Detail

Summary

• Symbol: Ank3^tm1Bnt
• Name: ankyrin 3, epithelial; targeted mutation 1, Vann Bennett
• MGI ID: MGI:2179751
• Synonyms: Ank3 exon 1b^-, ankG^-, cs-AnkG-KO
• Gene: Ank3 Location: Chr10:69234608-69863266 bp, + strand Genetic Position: Chr10, 36.1 cM
• Alliance: Ank3^tm1Bnt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51377
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 1b, which is an alternatively spliced exon found in transcripts only in brain. Northern blot analysis on RNA derived from homozygous brains demonstrated that no transcript containing exon 1b was expressed, while expression of transcripts containing other alternaltively spliced exons remained unaffected. Western blot analysis and immunohistochemistry experiments confirmed that little or no protein was seen in cerebellum. (J:51377)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ank3 Mutation: 170 strains or lines available

References

• Original: J:51377 Zhou D, et al., AnkyrinG is required for clustering of voltage-gated Na channels at axon initial segments and for normal action potential firing. J Cell Biol. 1998 Nov 30;143(5):1295-304
• All: 10 reference(s)