Ache^tm1Loc
Targeted Allele Detail

Summary

• Symbol: Ache^tm1Loc
• Name: acetylcholinesterase; targeted mutation 1, Oksana Lockridge
• MGI ID: MGI:2179948
• Synonyms: AChE-, AChE ko
• Gene: Ache Location: Chr5:137286516-137292728 bp, + strand Genetic Position: Chr5, 76.32 cM
• Alliance: Ache^tm1Loc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56242
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 5 kb genomic fragment containing exons 2-5 was replaced by a neomycin resistance gene cassette. Enzymatic activity assays on blood serum derived from homozygous mice confirmed that no functional protein was made from this allele. (J:56242)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ache Mutation: 30 strains or lines available

References

• Original: J:56242 Xie W, et al., Knockout of one acetylcholinesterase allele in the mouse. Chem Biol Interact. 1999 May 14;119-120:289-99
• All: 24 reference(s)