Xrcc6^tm1Fwa
Targeted Allele Detail

Summary

• Symbol: Xrcc6^tm1Fwa
• Name: X-ray repair complementing defective repair in Chinese hamster cells 6; targeted mutation 1, Frederick W Alt
• MGI ID: MGI:2179954
• Synonyms: Ku70-, Ku70DKO
• Gene: Xrcc6 Location: Chr15:81872036-81924286 bp, + strand Genetic Position: Chr15, 38.33 cM, cytoband E-F
• Alliance: Xrcc6^tm1Fwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44361
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 450 bp of this gene encompassing exon 4 and part of exon 5 were replaced with a neo cassette via homologous recombination resulting in a shift in the reading frame and predicted protein truncation after the first 64 residues. Homozygous mutant ES cells were identified by Southern blot analysis and Western blot analysis confirmed the absence of protein product. (J:41941, J:44361)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Xrcc6^tm1Fwa

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Xrcc6 Mutation: 63 strains or lines available

References

• Original: J:44361 Gu Y, et al., Growth retardation and leaky SCID phenotype of Ku70-deficient mice. Immunity. 1997 Nov;7(5):653-65
• All: 35 reference(s)