Grap^tm1Gsf
Targeted Allele Detail

Summary

• Symbol: Grap^tm1Gsf
• Name: GRB2-related adaptor protein; targeted mutation 1, Gen-Sheng Feng
• MGI ID: MGI:2179974
• Synonyms: Grap-
• Gene: Grap Location: Chr11:61544091-61563610 bp, + strand Genetic Position: Chr11, 37.96 cM, cytoband B2
• Alliance: Grap^tm1Gsf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76243
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An exon encoding amino acids 160-217 was replaced with a neomycin resistance cassette via homologous recombination. Southern blot analysis confirmed the targeted allele. Western blot of lymphocytes from homozygous mutant animals could not determine conclusively if the protein was absent or merely truncated. (J:76243)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grap Mutation: 16 strains or lines available

References

• Original: J:76243 Shen R, et al., Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction. Mol Cell Biol. 2002 May;22(10):3230-6
• All: 1 reference(s)