F8^tm1Kaz
Targeted Allele Detail

Summary

• Symbol: F8^tm1Kaz
• Name: coagulation factor VIII; targeted mutation 1, Haig H Kazazian Jr
• MGI ID: MGI:2179981
• Synonyms: E-16, FVIII KO, HemA, MFVIII-16
• Gene: F8 Location: ChrX:74216321-74426221 bp, - strand Genetic Position: ChrX, 38.17 cM, cytoband A7-B
• Alliance: F8^tm1Kaz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:24941
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 293 bp of sequence, including 7 bp at the 3' end of exon 16 and 286 bp at the 5' end of intron 16. Two different activity assays indicated that the mutated allele produced protein with levels of activity less than 1% of wild-type levels. (J:24941)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any F8 Mutation: 25 strains or lines available

References

• Original: J:24941 Bi L, et al., Targeted disruption of the mouse factor VIII gene produces a model of haemophilia A [letter]. Nat Genet. 1995 May;10(1):119-21
• All: 88 reference(s)